Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Phenolic Content, Antioxidant Capacity and Antimicrobial Activity of Essential Oil from Habbatus sauda Seed

Nur Farahin Ishak1, Noor Intan Shafinas Muhammad2, Nuraini Mohd Yusoff1, Sook Fun Pang1, Mohd Arman Abd Kadir1, Sazwani Suhaimi1, Hafizah Ramli1, Mohd Faried Abdullah1, Zainal Giman1 and Jolius Gimbun1,3*

1Faculty of Chemical & Natural Resources Engineering, 2Faculty of Technology, 3Center for Advanced Research in Fluid Flow (CARIFF), Universiti Malaysia Pahang, 26300 Gambang, Malaysia.

Received on 10 August 2014 and accepted on 31 October 2014



This paper presents an extraction and antimicrobial study of essential oil obtained from Nigella sativa seeds. Extraction using polar solvent resulted in higher recovery of desired bioactive compounds, thymoquinone and thymol. It was found that extraction of antioxidant (i.e. Agidol 7) favours aqueous solvent, i.e. 50% ethanol and 50% methanol. The highest TPC, TFC and antioxidant activities were obtained at power 224W and temperature of 50 ?C. This study shows that N. sativa seed oil has an effective antibacterial activity from the in-vitro studies. The result showed that the essential oil extracted using n-hexane has the highest (30.17%) effectiveness against E. coli.

Keywords : Nigella sativa, Ultrasonic assisted extraction, Flavonoid, Thymol, Thymoquinone, Antimicrobial.