Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Bio Evaluation of Different Fractions of Gazania rigens

Kommidi Devendar Reddy1*, Kandappa Himakar Reddy2, Moodley Brenda1, Koorbanally N.A.1 and Govender Patrick2

1School of Chemistry and Physics, 2Department of Bio Chemistry, University of KwaZulu-Natal, Westville, Durban - 4000, South Africa.

Received on 11 February 2014 and accepted on 15 April 2014

 

ABSTRACT

This communication describes the in vitro anti-bacterial, anti-fungal and anti-oxidative evaluation results of the different extracts of Gazania (Asteraceae). Whole plant was extracted using distilled n-hexane, DCM, ethyl acetate methanol and methanol: water subsequently. These fractions were further tested for in vitro anti-bacterial, anti-fungal and anti-oxidative activities using standard methods like micro broth dilution method for anti-bacterial and fungal, DPPH radical scavenging and H2O2 scavenging activity methods for anti-oxidative activity. The investigations resulted fractions with good anti-bacterial, anti-fungal and anti-oxidative activities, results were tabulated. Based on the results it was concluded that DCM, ethyl acetate, methanol and methanol: water fractions have good bio activity, these fractions may be further investigated for the pure compounds.

Keywords : Gazania, micro broth dilution, DPPH radical scavenging activity, H2O2 scavenging activity.