Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Biological Properties of Phytopathogenic Bacteria Pseudomonas syringae, Isolated from Sugar Beet

Katerina Dvorak1, Vasyl Sabluk1, Antonina Kalinichenko2, Lyudmyla Butsenko3, Lidiya Pasichnyk3 and Volodymyr Patyka3

1Institute of Bioenergy Crops and Sugar Beet of NAAS of Ukraine, 25, Klinichna str., Kyiv, 03141, Ukraine. 2Opole University, Process Engineering Chair, Poland, 45-365, Opole, Dmovskiego str, 7-9. 3D.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine (NAS), 154 Acad. Zabolotny St., Kyiv, 03680, Ukraine.

Received on 15 July 2014 and accepted on 30 August 2014

 

ABSTRACT

Pseudomonas syringae bacteria were isolated and identified from the sugar beet leaves with the characteristics of destruction. Their biological properties were determined and compared with collection strains of phytopathogenic bacteria that cause bacterial disease of this culture. The isolated strains caused the necrotic dark spot formation on the leaves upon artificial inoculation of sugar beet plants, which gradually increased in size, while the tissues inside of the spots got dry and fell out. We demonstrated that the lipids of bacteria cells, isolated from infected leaves of sugar beet, include fatty acids, which are typical for Pseudomonas syringae according to quantitative and qualitative composition. The studied strains showed serological relationship with antiserum against strains- pathovars P. syringae of various serogroups. The high titer of the investigated strains in agglutination reaction with antiserums against representatives of IV serogroup indicates their affiliation to the serogroup IV.

Keywords : Bacterial diseases, Isolates, properties, Identification, Pseudomonas syringae.