Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Optimization of Culture Medium for Polysaccharides Production of Antrodia camphorate using Response Surface Methodology

Yu Zhou, Fan Qing Sheng, Chen Juan, Jiang xinghua, Wang Jiang Lu, Shen Qi Rong and Fu Jin Heng

Sino-German Joint Research Institution, Nanchang University, #235 Nanjing East Road, Nanchang, 330047, P.R China.

Received on 09 September 2014 and accepted on 28 November 2014

 

ABSTRACT

Antrodia camphorate is preserved unique fungus which is an indigenous and rare mushroom found only in TaiWan only grows on a species of rain forest tree that is also native to TaiWan, which has been used in traditional Chinese medicine for more than 100 years. The main effective component of that is reported as polysaccharides. In this essay, the culture medium for increased polysaccharides were developed, soluble starch, wheat bran and MgSO4 were found to be the key factors of the culture medium according to the results obtained from the Plackett-Burman experimental design(PB).The optimal concentration range of the three factors was examined by the steepest ascent path. And Response surface methodology (RSM) was used to optimize culture medium. A Box?Behnken design(BB) was used for experimental design and analysis of the results to obtain the optimal culture medium.The 3-D response surface and the contour plots derived from the mathematical models were applied to determine the optimal culture medium.The optimum culture medium is soluble starch 3.17%, wheat bran 0.79%, KH2PO4 0.1%, MgSO4?7H2O 0.05%, VB1 10mg/100mL. The production has been raised to 183.46 mg/L, which increased 253% compared with 51.98 mg before optimization.

Keywords : Antrodia camphorate, Polysaccharides, Plackett-Burman experimental design, Steepest ascent path, Response surface methodology.