Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion

Valeriya V. Solovyeva1, Ilnur I. Salafutdinov1, Leysan G. Tazetdinova1, Svetlana F. Khaiboullina1,2, Ruslan F. Masgutov1,3 and Albert A. Rizvanov1

1Kazan Federal University, Kazan, Russia. 2Department of Biochemistry and Molecular Biology, University of Nevada, School of Medicine, University of Nevada-Reno, Reno, Nevada, USA. 3Republican Clinical Hospital, Ministry of Health of the Republic of Tatarstan, Kazan, Russia.

Received on 03 August 2014 and accepted on 31 October 2014

 

ABSTRACT

Bacterial plasmid DNA is often used in gene therapy application as a vector for delivering recombinant genes into eukaryotic cells. In this work we study effect of plasmid DNA encoding pro-angiogenic growth factors VEGF and FGF2 on cytokine production of human adipose derived stem cells (ADSC). Adipose tissue contain progenitor and stem cells with angiogenic potential which are considered promising cell source for treating ischemic conditions. Genetic modification of human ADSC with recombinant plasmids encoding therapeutic growth factors might change therapeutic potential of these cells. We report that genetic modification of ADSC with recombinant plasmid pBud-VEGF-FGF2 results in expected increased of VEGF secretion and also results in increase of IL-8 and MCP-1 secretion into culture medium.

Keywords : Bacterial plasmid DNA, Cytokines, Chemokines, Stem cells, Stromal vascular fraction, Vascular endothelial growth factor, Basic fibroblast growth factor, Genetic modification.