Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Selection of Potential Bacterial Bioremediator for Tiger Grouper (Epinephelus fuscoguttatus) Juveniles Culture

Noorashikin Md. Noor1, Wee Wen Chen1, Nur Izzati Ahmad1, Farha Fazidah1, Ina Salwany1,4, Murni Karim1,4, Sharr Azni Harmin3 and F.M.I. Natrah1,4*

1Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Selangor, Malaysia. 2 Faculty of Medicine, Universiti Putra Malaysia, 43400 UPM Selangor 3Faculty of Science and Biotechnology, Centre for Land and Aquatic Technology, Universiti Industri Selangor, 45600 Bestari Jaya, Selangor, Malaysia. 4 Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Selangor.

Received on 24 August 2014 and accepted on 09 October 2014

 

ABSTRACT

Different bacterial bioremediators were isolated from microalga Chlorella vulgaris and grouper culture water respectively. The total ammonia degradation test showed that all six strains degraded the total ammonia-nitrogen (TAN) in-vitro. One strain with the highest degradation activities, BP-GRP/2 was further tested in-vivo to tiger grouper, Epinephelus fuscoguttatus juveniles culture. No significant differences can be seen on TAN degradation and soluble reactive phosphorus (SRP) of the grouper culture water, except for the nitrite level was decreased in the presence of the strain. Higher survival of the fish in the BP-GRP/2 treatment was also observed compared to control. These results showed that certain bacterial strains from microalgae and grouper culture water can act as bioremediators and improve the survival of the fish host.

Keywords : Bioremediator, bacteria, microalgae and tiger grouper.