Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Prevalence and Antibiotic Susceptibility Profile of Pseudomonas aeruginosa Isolated from Different Clinical Samples in District Peshawar Pakistan

Irshad Ahmad

Biology Department, King Fahd University of Petrolium and Minerals (KFUPM), Dhahran, Post Code: 34464, Saudi Arabia.

Received on 25 May 2014 and accepted on 14 July 2014

 

ABSTRACT

Currently, antibacterial agents are widely used to treat bacterial infections. However, bacterial populations have adopted resistance mechanism against these antibacterial agents worldwide. Pseudomonas aeruginosa, which is a Gram-negative commensal of human microflora is one of the most common causative agents of nosocomial infections. Different types of clinical samples including pus, blood, urine, fluids and different swabs were collected from patients in district Peshawar, Pakistan. The pathogens were isolated by using sterile bacteriological media, including blood agar, MacConky agar, CLED agar and chocolate agar. All the clinical isolates of P. aeruginosa were tested for their sensitivity against antimicrobials including: amikacin (30 ug), ceftazidime (30 mcg), cefoxitin (30 ug), ceftriaxone (30 ug), cefipime (30 ug), ciprofloxacin (5 ug), co-amixoclave (30 ug), gentamicin (10 ug), imipenum (10 ug), meropenum (10 ug), sulzone (91 ug), fosfomycin (50 ug) and pipracillin-tazobactum (110 ug) of standard strengths respectively. We have observed the sensitivity profile of P. aeruginosa against Pipracillin-tazobatum is 90.4%, sulzon is 86.6%, amikacin is 82.8%, imipenum is 82.8%, meropenum is 80%, ceftazidime is 71.4%, fosfomycin is 69..5%, cefipime is 67.6%, cifrofloxacin is 71.6%, gentamycin is 63.8%, cefoxitin is 56.1%, co-amixoclave is 54.2% and ceftriaxone is 2.8% respectively. The results obtained herein indicate that a combination of pencilline+beta-lactamase inhibitors, imipenem and amikacin were the drugs of choice that can be used against multidrug resistant strains of P. aeruginosa.

Keywords : Pseudomonas aeruginosa, Prevalence, Antibiotic susceptibility.