Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Immunogenicity of Lipopolysaccharides from Pasteurella multocida Type B against Hemorrhagic Septicemia in Buffaloes

Asma Ashraf2, Shahid Mahboob1,2, Tayyaba Huma3 and Muhammad Salahuddin Shah4

1Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia. 2Department of Zoology, Wildlife & Fisheries, GC University Faisalabad, Pakistan. 3Department of Bioinformatics & Biotechnology, GC University Faisalabad, Pakistan. 4Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan.

Received on 11 July 2014 and accepted on 25 September 2014

 

ABSTRACT

Hemorrhagic septicemia (HS) is a fatal disease of cattle and buffaloes causing heavy economic losses. The causative organism (Pasteurella multocida) was isolated and identified from blood samples of infected animals on the basis of morphological and molecular characteristics. The lipopolysaccharides (LPS) were extracted by treating the cells with proteinase K and quantified following per iodic acid method. This LPS was mixed with Saccharomyces cerevisiae and different concentrations were injected subcutaneously in buffaloes. The OD values of ELISA showed that the antibody level against LPS was higher in Group D and it gradually decreased from 10th to 30th day post inoculation. Hence it was found that the LPS was immunogenic and its immunogenic activity can be improved by the addition of carrier proteins from S. cerevisiae.

Keywords : Pasteurella multocida, Hemorrhagic septicemia, LPS, ELISA, Immunity & Buffaloes.