Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Quantification of Amylase Enzyme from the Calcite Residing Actinobacteria Isolated from Limestone Quarries of Deccan Traps

Syed Raziuddin Quadri1,2,3, Jamal Al Ruwaili1, Shaik Mohammed Akbar5, Wen-Jun Li3,4 and Dayanand Agsar2

1Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar-91431, Northern Borders, Saudi Arabia. 2Department of Microbiology, Gulbarga University, Gulbarga-585106, Karnataka, India. 3Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, PR China. 4State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PR China. 5Entomology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Telangana State, India.

Received on 15 July 2014 and accepted on 09 September 2014

 

ABSTRACT

Sixty three actinobacteria were isolated from Limestone quarries of Deccan Traps from South India. The isolates were identified by morphological, biochemical and physiological studies. Among these, six isolates were selected on the basis of their amylolytic activity using submerged cultivation. The potential isolates exhibiting amylolytic activity were DRQ10, DRQ20, DRQ86, DRQ33, DRQ41, and DRQ63 as confirmed by formation of clear zones of starch hydrolysis around the colonies, with maximum activities 2500, 1100, 1200, 1150, 1250, 1400, and 1600 Units, respectively, as determined by DNS (3, 5-dinitrosalicylic acid) assay method. Various physicochemical parameters such as pH, temperature, incubation time were optimized for the production of amylase. Amylase production was maximum on 4th day of incubation. The optimum pH and temperature were found to be 9.0 and 45 oC, and NaCl concentration of 3M for the potential amylolytic isolates. The carbon and nitrogen source for the production of amylase was starch and peptone, respectively.

Keywords : Actinobacteria, Amylase, Limestone quarries, Deccan traps.