Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Mycoflora Colonization and Mycotoxin Accumulation in Cottonseeds

Abd El-Rahim M.A. El-Samawaty1,2, Medhat A. El-Naggar1,3, Mohamed A. Moslem2, Mohamed A. Yassin1,2* and Abdullah A. Al-Arfaj2

1Plant Pathology Research Institute, Agricultural Research Center. Giza, Egypt. 2Botany and Microbiology Department, College of Science, King Saud University. Riyadh, Suadi Arabia. 3National Research Central Lab., GSFMO, Saudi Arabia.

Received on 03 August 2014 and accepted on 26 October 2014

 

ABSTRACT

Cottonseed borne fungi were isolated from 5 cotton (Gossypium barbadense L.); Giza-80, Giza-86, Giza-88, Giza-90 and Giza-92. Alternaria spp., Aspergillus flavus, A. niger, Aspergillus spp. Fusarium spp., Penicillium spp., Rhizopus stolonifer and Trichoderma spp. occurred in both non-strilized and sterilized seeds. A. niger was generally the most predominant fungus with isolation frequency range of 20.95% to 73.17%. Mycotoxin analysis were performed by high performance liquid chromatography (HPLC). HPLC analysis of cottonseed revealed the presence of mycotoxin but the occurrence of each in the seeds varied with the genotype. Significant correlations between the frequency of isolated fungi and the mycotoxin production was recorded in this study. Seven models were constructed using the predictors supplied by stepwise regression with R² values ranged from 0.433 to 0.578%. Since the stepwise multiple regression was effectively constructed 7 predictive models; this study suggested that the contents of some mycotoxins in cotton seeds could be predicated depending on the fungal isolation frequencies.

Keywords : HPLC, Gossypium barbadense, Mycotoxins, Storage.