Journal of Pure and Applied MicrobiologyVol. 9 No. Special Edition May. 2015

Phytochemical Variation, Antimicrobial Activity and Genetic Diversity of Three Ulva Species Collected from Mediterranean Seashores of Alexandria, Egypt

Soad M. Mohy El-Din and Amani M.D. El Ahwany

Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.

Received on 18 January 2015 and accepted on 24 March 2015

 

ABSTRACT

Due to wide range of secondary metabolites and their therapeutic properties, seaweeds have natural alternative to the chemical based compounds. This project focuses on phytochemical evaluation and genetic diversity within seaweeds collected from Mediterranean seashores of Alexandria. The seaweeds samples of class Ulvophyceae such as U. fasciata, U. lactuca and U. intestinalis were extracted using polar solvents such as Methanol, Ethanol, Acetone and Chloroform. Phytochemical screening showed the presence of alkaloids, triterpenoids, steroids, tannins, coumarins, sterpenoids, phytosteroids and flavonoids in marine algae under investigation. Saponins were absent in all Ulva sp. The common major compounds like protein, carbohydrate, lipid, phenol, flavonoids, tannins and photosynthetic pigments were extracted using polar solvent methanol. Of the four solvents tested, Methanol was the best solvent for isolation of antimicrobial compounds from the tested marine algae followed by Ethanol. The reducing power, percentage of hydrogen peroxide scavenging and total antioxidant capacity of the various extracts were determined. Among the various extracts methanolic extract was found to have the highest reducing power and total antioxidant capacity, while chloroform extract was found to have highest of hydrogen peroxide scavenging activity. The present study elaborates the bioactive content of seaweeds and demonstrates the effective application of RAPD technique for analyzing the genetic differentiation.

Keywords : Algae, Phytochemical screening, Antibacterial activity, RAP.