Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Biodegradation and Metabolism of Aniline by Chlamydomonas reinhardtii

Chi Zhu1,2*, Bing Li1, Shui Wang1, Wenjie Chang1 and Weixiang Wang1

1Jiangsu Provincial Academy of Environmental Science, Jiangsu provincial key laboratory of environmental engineering, Nanjing 210036, China. 2State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China.

Received on 30 August 2014 and accepted on 19 October 2014

 

ABSTRACT

The biodegradation and responses of detoxification enzymes in the freshwater green algae, Chlamydomonas reinhardtii, upon exposure to 10 mg/L concentration of aniline were investigated. Results showed that aniline was metabolized, and bioaccumulation and adsorption were 1.2%. The removal of aniline over an 8-day period in the control medium and with the group of ABT was 32% and 35%, respectively. With algae culture the removal of aniline was 56%. About up to 24% of total aniline was biodegraded or biotransformed by C. reinhardtii in 8 days. C. reinhardtii biotransformed aniline into acetylaminobenzene and the cytochrome P-450 was the key enzyme in the metabolic pathway.

Keywords : Aniline; Degradation; Chlamydomonas reinhardtii; Detoxification; Enzyme; N-acetylation.