Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Optimization of the Medium Composition using Response Surface Methodology for Production of Trehalose Synthase from Corynebacterium glutamicum

Maohua Qu, Juan Du and Weiping Chen*

Key Lab of Agricultural Products processing and quality control of Nanchang City, Jiangxi Agricultural University, Nanchang - 330045, China.

Received on 12 June 2014 and accepted on 14 August 2014

 

ABSTRACT

The medium was optimized for trehalose synthase production by Corynebacterium Glutamicum. Effects of factors on trehalose synthase production were examined to improve trehalose synthase yield. Experimental designs were applied to optimize medium. Results of factional factorial design (FFD) showed that maltose, NaCl, K2HPO4 and beef extract were the most significant factors affecting trehalose synthase production. Further central composite design (CCD) experiments showed that optimal maltose and NaCl concentrations were 22g/L and 13.36g/L, respectively. Trehalose synthase activity increased to 580 U/mL, an approximate 1.76-fold increase over the previous activity (330 U/mL) using an optimized fermentation medium with the main composition maltose (22g/L), beef extract (7.49g/L), NaCl (13.36g/L), K2HPO4(11.33g/L) and peptone (15.00g/L).

Keywords : Trehalose synthase, Optimization, Response surface methodology.