Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Fungal Endophytes Survey of Some Legume Seeds

Abd El-Rahim M. A. El-Samawaty1,2, Mohamed A. Moslem2, Shaban R. M. Sayed2 and Mohamed A. Yassin1,2*

1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. 2Botany and Microbiology Department, College of Science, King Saud University. Riyadh, Saudi Arabia. 3Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

Received on 15 September 2014 and accepted on 24 October 2014

 

ABSTRACT

Mycological survey of associated fungi with common bean (Phaseolus vulgaris) and cowpea (Vigna unguiculata) seed samples, collected from markets throughout the Riyadh region of Saudi Arabia, was investigated. Frequencies of the isolated fungi were statistically compared. Seventeen and sixteen species belonging to eleven genera were isolated from common bean and cowpea respectively. Aspergillus niger and A. flavus were the highest distributed species (55.55%) over common bean sample locations. A. niger was also the highest distributed species (61.11%) over cowpea sample locations followed by A. flavus (58.33%) and Fusarium spp. (50%). A. niger was the most dominant in both crop seeds with 22.09% and 26.83% isolation frequencies of the total fungi of common bean and cowpea respectively. A. flavus was second and third most frequent fungus of the total fungi isolated from common bean (21.12%) and cowpea (18.48%) respectively. Significant positive and/or negative correlation were among some isolated fungi, implying the potential existence of synergism and/or antagonism among these fungi. Thus, more effort should be made to minimize the risk of endophytes accumulation in imported agricultural commodities.

Keywords : Phaseolus vulgaris, Myco-contaminant, Vigna unguiculata, Pulses.