Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Optimization of Cellulase Producing Aspergillus flavus SB4 by Solid State Fermentation using Rice Bran

N. Utharalakshmi1, A. Ganesh Kumar2 and G. Narendrakumar 1

1Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai - 600119, India. 2National Institute of Ocean Technology, NIOT Campus, Chennai - 600 100, India.

Received on 06 September 2014 and accepted on 13 October 2014

 

ABSTRACT

This study was aimed to isolate and screen the cellulolytic ability of fungi from litter soil samples collected in and around Chennai, India. The culture conditions were optimized for the growth of fungi by SSF using Rice Bran. Fungi are the main cellulase producing microorganisms when compared with other microorganisms. Among eleven fungal isolates, a fungal isolates were identified as maximum cellulase producer and labelled as SB4. The cultures were maintained on PDA slants at 4°C. The morphology was studied by LPCB method and fungal identification was done based on 18s rRNA ITS sequencing. The zone of clearance was observed on CMC agar plates after 72 hours of incubation by staining with 1% Congo red. The zone of hydrolysis was found to be high for SB4 having the highest diameter of 17 mm. Maximum cellulase production for rice bran was observed to be high at initial moisture 85%, pH-6, temperature-31°C and fermentation period was 14th day. The enzyme production was analyzed individually by Dinitrosalicylic acid (DNS) method. The cellulosic degradation was confirmed by FTIR and SEM on 0th day and 28th day.

Keywords : Cellulase, Aspergillus flavus, Solid state Fermentation, FTIR, SEM.