Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Decolorization of Azo Dye by Enteropathogenic Eschericha coli Strains Isolated from Wastewater

E. Chavez Bravo1, G. Valencia Toxqui2, J.A. Rivera Tapia1, E.I. Castaneda Roldan1 and A.I.A. Alonso Calderon3

1Center for Research in Microbiological Sciences-Institute of Sciences Autonomous University of Puebla, Mexico. 2School of Biology BUAP, 3Faculty of Chemical Engineering, BUAP.

Received on 18 August 2014 and accepted on 22 October 2014



The presence of enteropathogenic E. coli (EPEC) in wastewater represent a public health problem because it is causative agent of outbreaks diarrheal, the interaction between this pathogens and chemical pollutants in wastewater effluent it is poorly understood, nevertheless it is well known that decolorization of dyes resulted in the formation of intermediate metabolites could be more toxic that dyes, the objective of this study was recovered from wastewater enteropathogenic E. coli strains able to decolorize azo dye. Samples of wastewater were collected and examined for identification and characterization of EPEC pathotype by PCR, after were quantified percent of decolorization the azo dye Direct Black 22 at 200 and 300 ppm for 10 days, and finally performed partial characterization of reaction products. A total of 106 E. coli strains isolated in this study, 66 (62.02%) were characterized into pathotype (EPEC) and were able to decolorize azo dye to 200 and 300 ppm. In this study evidenced a double concern about presence of EPEC, first leads to the risk of acquisition of diarrheal disease and second per possible participation in water chemical pollution toxic metabolites generated from the decolorization of azo dye.

Keywords : Decolorization, Azo dye, E. coli, Wastewater.