Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Mortality Evaluation of Armyworm (Spodoptera frugiperda J. E. Smith) by using Metarhizium anisopliae In vitro

O. Romero-Arenas1, 2, A. Rivera3, 2, A. Aragon1,2, C. Parraguirre4, E. Cabrera4 and F. Lopez1,2

1Center for Agroecology, Autonomous University of Puebla, Mexico. 2Postgraduate Megree in Sustainable Agroecosystem Management, Autonomous University of Puebla. 3Center for Research in Microbiological Sciences, Autonomous University of Puebla, Mexico. 4Regional Unit Tetela, Autonomous University of Puebla, Mexico.

Received on 20 September 2014 and accepted on 28 October 2014

 

ABSTRACT

The biological control use to reduce pest insect populations is an alternative to the use of synthetic insecticides technique. The use of entomopathogenic fungi and microbial control has some advantages over the use of chemical insecticides, which emphasizes the high specificity of the host and low environmental contamination. However, the production of biological control agents such as Metarhizium anisopliae, presents constraints on the optimal concentration of conidium, the potential well may have unknown native strains. It was evaluated in this research, the mortality of fall armyworm (Spodoptera frugiperda J. E. Smith) in laboratory conditions by using concentrations of conidium from a native strain and a commercial strain of M. anisopliae. Different suspensions of conidium were conducted where 53x104, 9x104 were obtained and with 5x104 / mL of native strain of M. anisopliae and 36x104, 7x104, and 4x104 con/mL of commercial strain of Metarhizium. The concentration of 53x104 con/mL of the native strain, reported a mortality of 72.5% and the lowest was obtained from the strain of Metarhizium commercial in concentration of 4x104 con/mL, equivalent to 32.5%. The highest death rate for both strains occurred at 72 hrs. post-infection with 11 larval on average of three concentrations of native Metarhizium and 6 commercial strains larval. These results demonstrate the feasibility of using M. anisopliae for biological control armyworm (S. frugiperda) in vitro.

Keywords : Biological control, Native strain, Conidium concentration and Mortality rate.