Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Diversity and Isolation of Culturable ACC Deaminase-Producing Rhizobacteria from the Red-crowned Crane National Nature Reserve, China

Min Shen1,2†, Feibao Wang2†, Daocheng Cheng2 and Yijun Kang1,2*

1Jiangsu Key Laboratory for Bioresources of Saline Soils, Yancheng, Jiangsu 224051, P. R. China. 2School of Life Science and Technology, Yancheng Teachers University, Yancheng, Jiangsu 224051, P. R. China.

Received on 15 June 2014 and accepted on 21 October 2014

 

ABSTRACT

This study aimed to assess the richness and diversity of ACC deaminase-producing bacteria (ADPB) in native plant rhizospheres, and also to screen ADPB isolates for further development. In September 2013 (summer) and February 2014 (winter), we sampled three types of plants Saudeasalva (SS), Imperata koenigii (IK), and Phragmites australis (PA) with distinct salt-tolerant abilities from the reserve. Using enrichment culture method together with amplifed ribosomal DNA restriction analysis technology, an average of 23.25 (20.5), 23.75 (13.75), and 21.50 (11.75) ADPB were screened out corresponding to SS, IK, and PA in summer (winter). 16S rRNA gene sequence data showed that there were 5 and 8 OTUs in summer and winter respectively, in which, Klebsiella was the absolutely dominant genus, while before enrichment, Pseudomonas was the dominant genus as revealed by PCR-DGGE. The comparison of ADPB communities among three plants reflected that there is no correlation between ADPB population in rhizosphere and salt-tolerant abilities. Two representative isolates of each OUT were determined for the possible plant growth-promoting traits, among which, Sphingobacterium sp. is the first reported isolate with the ability of producing ACC deaminase. Surprisingly, all isolates were gram-negative, suggesting that most ADPB in nature may belong to Gram-negative group.

Keywords : ACC deaminase-producing bacteria (ADPB); Amplifed ribosomal DNA restriction analysis (ARDRA); Enrichment culture; The Red-crowned Crane National Nature Reserve.