Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Improvement of Trehalose Biosynthesis in Corynebacterium glutamicum by a Combination of Conventional Mutagenesis and Analysis of Enzymatic Properties

Maohua Qu, Juan Du, Fengying Zhang, Mingfang He and Weiping Chen*

Key Lab of Agricultural Products Processing and Quality Control of Nanchang City, Jiangxi Agricultural University, Nanchang, 330045, China.

Received on 23 June 2014 and accepted on 09 August 2014

 

ABSTRACT

Corynebacterium glutamicum mutant strain with higher yield of trehalose was obtained using UV-chemical mutagenesis. The yield increased to 284.4% compared to wild strain and then studied the enzymatic properties of five synthases for trehalose. The optimum pH value for trehalose synthase, MTSase, MTHase, TPS and TPP was 7.0, 7.5, 6.5, 6.5, 7.5 and the range of pH 6.5-8.0, pH 6.5-7.5, pH 6.0-7.5, pH 6.0-7.5, pH 6.5-8 with over 80% of highest activity, respectively. The optimum reaction temperature for them was 35°C, 35°C, 40°C, 35°C, 35°C with maintaining higher reactivity at the range of 30-35°C, 35-40°C, 30-45°C, 25-40°C, 30-35°C, respectively.

Keywords : Trehalose, mutagenesis, enzymatic property.