Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Secretion Expression of Recombinant Stichopus japonicus Lysozyme in Bacillus subtilis WB600

Liu Zhiwen, Xu Xiaomin, Liao Xingyu, Sun Lu, Zou Dan, Li Dan and Cong Lina*

School of Biological Engineering, Dalian Polytechnic University, Dalian Liaoning, P.R. 116034, China.

Received on 20 August 2014 and accepted on 30 October 2014

 

ABSTRACT

In this study, a genetic engineering bacteria Bacillus subtilis, pHT43-SjLys/WB600, was successfully constructed for the expression of the lysozyme gene from sea cucumber (Stichopus japonicus). The growth rate of engineering bacteria was consistent with that of wild type strain, WB600, and the presence of lysozyme did not affect its growth. The stability analysis showed that there was no gene rearrangement and lost of the recombinant plasmid in the bacteria which showed that it has high genetic stability. Soluble SjLys was successfully expressed with 1 mmol/L IPTG treatment for 36, 48 h and the soluble protein showed remarkable inhibitive effect on the growth of the Vibrio parahaemolyticus. To our knowledge, this is the first report of the SjLys gene authentic heterologous expressed in Bacillus subtilis and it may provide a new way for recombinant SjLys production by this secretion system.

Keywords : Sea cucumber lysozyme, Bacillus subtilis, Genetic engineering bacteria, Antimicrobial activity.