Journal of Pure and Applied MicrobiologyVol. 8 No. Special Edition Nov. 2014

Evaluating the Antibacterial Activity of Fenugreek (Trigonella foenum-graecum) Seed Extract against A Selection of Different Pathogenic Bacteria

Mona S. Alwhibi* and Dina A. Soliman

Department of Botany and Microbiology, King Saud University, P. O. Box: 22452, Riyadh-11495, Kingdom of Saudi Arabia.

Received on 18 September 2014 and accepted on 28 October 2014

 

ABSTRACT

In the present study, Fenugreek plants obtained from two different cultivars, one from Saudi Arabia and another from Yemen, were screened for phytochemical active constituents and investigated for antimicrobial properties against a selection of gram-positive and gram-negative pathogenic bacteria. Five different solvent seed extracts from each cultivar were tested. The results of the study demonstrated that the chloroform and methanolic extracts possessed significant antibacterial activity against Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC25923, Salmonella typhi ATCC14027 and Klebsiella pneumonia ATCC700603. The antimicrobial activity of the extracts was investigated using the agar well diffusion method. The results of the antimicrobial analysis identified Shigella sonnei as the most sensitive pathogen to the crude extracts of Fenugreek seeds obtained from Saudi Arabia and with the largest zones of inhibition.

Keywords : Trigonella foenum-graecum, Seed Extrac Abstract, Antibacterial Activity.