Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Prevalence Study of Brucella melitensis and Brucella abortus in Cow?s Milk using Dot Enzyme Linked Immuno Sorbent Assay and Duplex Polymerase Chain Reaction

Farhad Safarpoor Dehkordi*, Yousef Valizadeh, Tannaz Alipour Birgani and Khashayar Ghasemi Dehkordi

Young Researchers and Elites Club, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.

Received on 12 July 2013 and accepted on 29 August 2013

 

ABSTRACT

Ingestion of raw infected milk and dairy products is the most important way for human brucellosis. This study was carried out in order to investigate the prevalence of Brucella spp., Brucella abortus and Brucella melitensis in cow?s milk using culture, dot-ELISA and duplex PCR. All of the 150 milk samples were cultured for Brucella spp. and the positive results have been evaluated for presences of Brucella abortus and Brucella melitensis using dot-ELISA and duplex PCR assays. Totally, 12% of milk samples were positive for Brucella spp. using culture. The dot-Elisa assay indicated that 12% and 2% of milk samples were positive for Brucella abortus and Brucella melitensis, respectively. Novel duplex PCR showed that 14%, 4% and 2% of milk samples were positive for Brucella abortus, Brucella melitensis and both bacteria. Isfahan had the highest incidence of Brucella spp. (17.5%), while Chaharmahal Va Bakhtiari had the lowest incidence of Brucella spp. (8%). There were significant differences about P < 0.05 for incidence of Brucella spp. between Isfahan with Chaharmahal Va Bakhtiari provinces. Our study showed that milk inspection should be performed in order to diminish the distribution of brucellosis in human populations.

Keywords : Brucellosis, Milk samples, Culture, Dot-ELISA, Duplex PCR.