Journal of Pure and Applied MicrobiologyVol. 8 No. 2

A Comparison of Loop-Mediated Isothermal Amplification (LAMP) with PCR and Rapid Urease Test (RUT) to Detect Helicobacter pylori in Biopsy Samples

Parastoo Chamanrokh1*, Mohammad Hassan Shahhosseiny2,3, Mahnaz Mazaheri Assadi4, Taher Nejadsattari1 and Davoud Esmaili5

1Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran. 2Department of Microbiology, Islamic Azad University, Qods Branch, Tehran, Iran. 3Iranian Gene Fanavar institute (IGF), Tehran, Iran. 4Environmental Biotechnology Group, Biotechnology Department, Iranian Research Organization for Science and Technology,Tehran, Iran. 5Applied Microbiology Reasearch Center, and Microbiology Department, Baqiyatallah University Medical of Science, Iran.

Received on 18 July 2013 and accepted on 31 September 2013

 

ABSTRACT

Helicobacter pylori is one of the most successful pathogens and the source of morbidity of about half of the worldwide population. Detection of these bacteria using traditional methods has some difficulties due to existence of some coccoid forms. The aim of this study is to compare different diagnostic methods such as LAMP, RUT and PCR in the patient biopsy samples. In this research, DNA of standard H. pylori extracted by DNG method. The LAMP and PCR were optimized and sensitivity and specificity of tests were studied. Then, 100 biopsy samples were obtained. RUT was done on all the biopsy samples. DNA extraction was done using DNP kit for all patient samples. Then the samples were studied using PCR and LAMP tests. The PCR test sensitivity was obtained 10 per reaction and LAMP test sensitivity was 5 per reaction. No undesirable products were observed in specificity test with DNA samples. 64% of the stomach biopsy samples were positive using RUT and 76% by PCR and 87% by the LAMP. The results indicate LAMP test has higher accuracy, sensitivity and specificity compared to PCR and RUT, therefore LAMP technique could be used as an alternative method in the H. pylori detection especially in developing country.

Keywords : Helicobacter pylori, RUT, PCR, LAMP.