Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Pathogenicity of Fusarium oxysporum f.sp. melonis to Melon Genotypes (Cucumis melo L.) and Its Biocontrol

Mohamed El-Sheshtawi1, Ali H. Bahkali2, Wafa?a. A. Al-Taisan3 and Abdallah M. Elgorban2,4*

1Plant Pathology Department, College of Agriculture, Mansoura University, Mansoura 35516, Egypt. 2*Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. 3Department of Biology, College of Science, University of Dammam, P.O. Box 838 Dammam 31113 Saudi Arabia. 4Plant Pathology Institute, Agricultural Research Center, Giza, Egypt.

Received on 23 March 2014 and accepted on 06 May 2014

 

ABSTRACT

Fusarium wilt in melon (Cucumis melo L.) is widespread, responsible for serious economic losses in yield of melon in Egypt. Laboratory and greenhouse experiments were performed to evaluate the effects of different biocontrol agents (BCAs) against Fusarium oxysporum f.sp. melonis. In vitro, Trichoderma viride and Gliocladium virens significantly reduced the mycelia growth of F. oxysporum f.sp. melonis that produced 86.33 and 86.11% reduction, respectively. Under greenhouse conditions, 1022, caruso, primal and ideal genotype were the best genotypes for tolerant the infestation by F. oxysporum f. sp. melonis by 60% survival plants when compared with other genotypes. On the other hand, all BCAs tested significantly decreased the disease incidence of F. oxysporum f.sp. melonis. The treatment with T. viride completely suppressed the disease incidence after 15 days from sowing giving 100% survival plants and 96% living plants after 45 days.

Keywords : Genotype, Biocontrol agents, Fusarium wilt, Melon.