Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

In-vitro and in-vivo Anticoccidial Activities of Berberine against Murine Eimeria papillata Infection

Nour E. Sherif1, Mahmoud S. Metwaly1,2, Saleh Al-Quraishy2 and Mohamed A. Dkhil2,3*

1Department of Zoology, Faculty of Science, Suez Canal University, Ismailia, Egypt. 2Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. 3Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.

Received on 15 March 2014 and accepted on 14 May 2014

 

ABSTRACT

Intestinal Eimerian infections are considered to be one of the most highly distributed protozoan parasitic infections among farms and domestic animals, and cause huge economic losses in the field of animal farming and meat production. All recent trends to fight infectious diseases depend upon using treatment of natural origins instead of synthetic drug therapy. Berberine is a plant derived isoquinoline alkaloid with wide spectrum pharmacological and antimicrobial activities. The current study was designed to elucidate the in-vitro and in-vivo anticoccidial activities of berberine chloride against murine intestinal Eimeria papillata infection. In-vitro measurements showed that berberine could significantly reduce the viability and sporulation percent of E. papillata unsporulated oocyst. In addition, berberine showed a pronounced in-vivo anticoccidial activity as revealed by reducing the number of zygots and developing oocysts within intestinal villi by about 90% and that of schizonts and gamonts by 58% and 30% respectively. Moreover, berberine treatment of E. papillata infected mice showed a great increase in the total antioxidant capacity within jejunum tissue. Finally, the results of the study proved both in-vitro and in-vivo anticoccidial activities of berberine chloride against the murine intestinal E. papillata infection, and could effectively correct the induced disruption in the antioxidant capacity within jejunum tissue by such infection.

Keywords : Eimeria papillata, berberine, anticoccidial, in-vitro, in-vivo.