Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Genetics of Alkane Degrading Enzymes by Pseudomonas frederiksbergensis

Ahmed Abdel-Megeed1,2, Al-Meshal Areej Suliman3 and Rudolf Mueller4

1Department of Plant Protection, Faculty of Agriculture, (Saba Basha), Alexandria University, Egypt. 2Botany and Microbiology Department, College of Science, King Saud University, P. O. Box 2455 ? Riyadh 11451, Saudi Arabia. 3Department of Biology, College of Science &Humanity Studies, Salman bin Abdulaziz University, P.O.Box 292 AlKharj 11942, Saudi Arabia. 4Institute of Technical Biocatalysis, Technical University Hamburg-Harburg, Denickestr. 15, D-21073 Hamburg, Germany.

Received on 24 January 2014 and accepted on 19 March 2014

 

ABSTRACT

P. frederiksbergensis alkane monooxygenase was cloned in order to increase the understanding of the enzyme system involved in aerobic alkane degradation. The sequence comparisons and ORFS of DNA fragment results revealed that the regulation and the genes organization involved in alkane oxidation represented in Pseudomonas frederiksberghensis varies among the different known alkane degrading bacteria. Such genomes encode messages on both strands of the DNA, or in an overlapping but different reading frames, of the same strand of DNA. The gene culster of P. frederiksbergensis contained alkane monooxygenase and putative flavin-binding monooxygenase (hypothetical protein with unknown function) which was oriented in the same direction. Alcohol dehydrogenase oriented in the opposite direction.

Keywords : Alkane degrading enzymes, Pseudomonas frederiksbergensis.