Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Evaluation of Antiviral Activity of Berberine against Herpes Simplex Viruses

Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1

1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. 2Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.

Received on 17 March 2014 and accepted on 21 April 2014



The different nucleoside analogues such as acyclovir are effective antiviral drugs against herpes simplex virus infections since its introduction. However, with the emergence of acyclovir-resistant, there is a need to develop an alternative antiherpetic drug and natural products could be the potential lead. In this study,?the antiviral properties of berberine was evaluated against?herpes?simplex virus?types?1 and 2 (HSV-1, 2) in vitro. HSV-infected Vero cells and cell-free virus suspensions were treated with berberine, and virus yield and infectivity were quantified by direct plaque assay. The results of the present study showed that berberine at 150 ?g/ml provided 76.5% inhibition of plaque of HSV-1 and 80% inhibition against HSV-2. These properties suggest that this alkaloid could provide advantage as a topical prophylactic/therapeutic agent for herpes infections.

Keywords : Berberine, Herpes simplex virus; Antiviral.