Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Production and Purification of Lipase from Pseudomonas sp. AB2 with Potential Application in Biodiesel Production

Emad A. Abada1,2

1Department of Botany and Microbiology, Faculty of Science, Helwan University, Ain Helwan, Cairo, Egypt. 2Department of Biology, Faculty of Science, Jazan University, Jazan, KSA.

Received on 20 March 2014 and accepted on 08 May 2014

 

ABSTRACT

Lipase produced from microorganisms can be used in many industrial applications, such as detergent formulation, oil/fat degradation, pharmaceutical synthesis, biodiesel and cosmetics production. Pseudomonas sp. AB2 was isolated and identified by 16SrRNA from waste cooking oil (WCO). The lipase showed a molecular weight of 28.6 KDa by SDS?PAGE. The enzyme activity was highest after 24 h at 200 rpm agitation when (4X104 CFU) was used as inoculums. The maximum lipase production was observed at 35?C and pH 7. As the Tributyrine increase the enzyme activity decrease, while the yeast extract increase the activity with concentration higher than 0.5 g/l. The GC analysis of the WCO showed that the fatty acid profile of the oil was Palmetic (31.79%), Stearic (2.41 %), Oleic (65.8 %); while the total saturated acid was (34.2 %) and unsaturated acid was (65.8%). Pseudomonas sp. AB2 lipase, which exhibited a potential for catalyzing the biodiesel production, was further purified and immobilized on 20.4 mmol of Tetramethoxyorthosilicate (TMOS). The immobilized lipase from Pseudomonas sp. AB2 could be used as a biocatalyst for the application of enzyme-catalyzed biodiesel synthesis

Keywords : Waste cooking oil, Pseudomonas sp., Lipase, TMOS, Biodiesel.