Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Phenotypic, Histopathologic and Genomic Diagnosis of A Novel Fish Pathogen Vagococcus salmoninarum in Turkey

Ifakat Tulay Cagatay1* and Erkan Gumus2

1Akdeniz University, Faculty of Fisheries, Department of Basic Sciences, Molecular Microbiology Section, Campus, 07058, Antalya, Turkey. 2Akdeniz University, Faculty of Fisheries, Department of Aquaculture, Campus, 07058, Antalya, Turkey.

Received on 28 January 2014 and accepted on 06 March 2014

 

ABSTRACT

In the present study, the aim was to diagnose Vagococcus salmoninarum by using phenotypic, histopathologic and genomic methods together. Vagococcosis is a novel and emerging disease in rainbow trout (Oncorhynchus mykiss) farms and outbreak of the disease results in considerable economic losses to the aquaculture industry worldwide. The sixty bacterial isolates from fish tissues which had widespread infection were identified and characterized as V. salmoninarum by studying the cultural pattern and biochemical-enzymatic traits with BBL CrystalTM identification system. Among sixty phenotypical positive isolates, only 30% percent were verified as V. salmoninarum by PCR amplification of bacterial 16S rDNA gene. Further, observed spots of bacterial accumulation in histological sections of heart and kidney were subsequently diagnosed as V. salmoninarum with 16S rDNA gene amplification.

Keywords : Vagococcus salmoninarum, Rainbow trout, PCR, histopathology.