Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Evaluation of Extracts from Leaves of Brachychiton diversifolius R.Br against the Growth of Some Clinical Pathogens

Mohamed Z.M. Salem1, Mohamed H. Khamis5, Nader A. El-Shanhorey2, Mohammed A. Al-Muwayhi3, Mohmmad K. Okla4 and Hayssam M. Ali4,5*

1Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt. 2Department of Botanical Gardens Research, Horticultural Research Institute (ARC), Alexandria, Egypt. 3Department of Physics and Chemistry, Faculty of Science, Shaqra University, P.O. Box 33, Shaqra, 11961, Kingdom of Saudi Arabia. 4Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. 5Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Agriculture Research Center, Alexandria, Egypt.

Received on 08 January 2014 and accepted on 24 March 2014

 

ABSTRACT

In the present study the acetone, methanol, chloroform, ethanol and distilled water extracts of leaves from Brachychiton diversifolius R.Br were evaluated for their antibacterial activity against four clinical pathogens namely; Micrococcus luteus, Staphylococcus aureus, Acinetobacter baumannii and Pseudomonas aeruginosa. The inhibition zones (IZs) were ranged between 7-17 mm at a concentration of 2000 ?g/mL. Methanol and ethanol extracts showed good activity against the growth of M. luteus (IZ value of 17 mm). Chloroform extract presented moderate activity against S. aureus (IZ value of 8 mm). The methanol extract presented good activity against A. baumannii (IZ value of 15 mm) followed by the chloroform extract (IZ value of 12 mm). Furthermore, the distilled water extract was showed a selectively active against the growth of P. aeruginosa with IZ value of 10 mm, while the other extracts did not show any activity. The present results could be promise a basis for further work related to the bioactivity agents from B. diversifolius against the growth of human bacterial pathogens.

Keywords : Brachychiton diversifolius R.Br; Extracts; Antibacterial activity; Clinical pathogens.