Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Endophytes Community of Populus euphratica Oliv and the Strains Improving the Salt Tolerance of Plant

Xiangyang Ju1, Liming Ouyang1* and Lili Zhang2

1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. 2Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar 843300, China.

Received on 13 February 2014 and accepted on 19 April 2014

 

ABSTRACT

The diversity of endophytic bacteria associated with wild forest of Populus euphratica Oliv located in arid and saline area of northwestern China was investigated for the first time. 197 stable isolates were isolated from the tissues of P. euphratica trees and were further classified into 102 operation taxonomic units (OTUs) by amplifed ribosomal DNA restriction analysis (ARDRA) and morphology. Based on the sequence analysis of partial 16S rRNA genes, the 102 representative strains of each OTU belong to 21 genera, in which the Bacillus and Pseudomonas were the most common consisting of 49.0% and 14.7% of the community respectively. 16.7% of the representative strains were demonstrated to improve the germination of wheat (Triticum aestivum) seeds under salinity stress as plant growth promoting bacteria (PGPB). However, most lost the germination promoting effects when salinity stress was revoked. Interestingly, the proportion of PGPB on all endophytes exhibited a marked escalating trend along with the increase of bacterial salt tolerance.

Keywords : Endophytes; Bacterial community; Salinity stress; Populus euphratica; PGPB; ARDRA.