Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Characterization of Phage PA26 Infecting Pseudomonas aeruginosa

Min Soo Kim1,2, Young Deuk Kim1, and Heejoon Myung1,2*

1Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yong-In, Gyung-Gi Do 449-791, Korea. 2The Bacteriophage Bank of Korea, Yong-In, Gyung-Gi Do 449-791, Korea.

Received on 21 January 2014 and accepted on 06 April 2014

 

ABSTRACT

In this study we isolated a novel bacteriophage PA26 infecting Pseudomonas aeruginosa from a water reservoir. PA26 belongs to the Myoviridae family. The genome is a double stranded DNA of 72,321 basepair. Eighty eight predicted open reading frames were found, of which fourteen showed functional homology to known proteins. 99% of the phages were adsorbed to the host within 10 minutes after infection. The eclipse period was 10 minutes, and the latent period was 15 minutes, with aburst size of 252. Infectivity was highest at pH 7 and remained stable at temperatures between 4 to 37 oC. The phage inhibited biofilm formation by P. aeruginosa and also removed preformed biofilm.

Keywords : PA26, bacteriophage, Pseudomonas aeruginosa, biofilm, phage therapy.