Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

The Study of Cell Attachment and Spreading on Polyaniline and Gelatin using Electric Cell-substrate Impedance Sensing

Irmanisha Ibrahim1, Anis Nurashikin Nordin2* and Yumi Zuhanis Has-Yun Hashim1

1Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia. 2Department of Electrical and Computer Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia.

Received on 08 January 2014 and accepted on 24 March 2014

 

ABSTRACT

Electrical cell-substrate impedance sensing (ECIS) is a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. This study investigates the correlation between cell attachment and impedance when cells are attached to two different substrates, polyaniline and gelatin, respectively. Colon cancer cell lines, HCT-116 were used as model cell line. The impedance measurements were measured every 8 hours for 104 hours at frequency of 40Hz to 10MHz using impedance analyzer. Polyaniline produced a graph that is in agreement with typical growth curve for mammalian cell culture at lag, and early log phase. However, gelatin graph showed a different trend. This may be due to the high conductivity of the polyaniline which gave better cell attachment and spreading for the HCT-116 cells. The efficiency of the biosensor was measured by cytotoxicity test using 2.5 ?g/ml 5-FU and the changes on impedance were analyzed. In conclusion, the cell attachment correlates with impedance depending on the substrate used to culture the cells. To this end, ECIS is proven as an alternative tool to measure cell behavior with an added advantage of ability to monitor the progress in real-time and showed a great potential in drug testing application.

Keywords : ECIS; Impedance Biosensor; Drug testing; HCT-116; Cell Attachment.