Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Optimization of Media Composition for a-amylase Production in Liquid State Fermentation of Bitter Cassava by Aspergillus niger

Abubakir I. Khalaf, Md. Z. Alam* and Hamzah M. Salleh

Bioenvironmental Engineering Research Centre (BERC), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University, (IIUM), 53100 Kuala Lumpur, Gombak, Malaysia.

Received on 08 January 2014 and accepted on 24 March 2014

 

ABSTRACT

A number of media components influencing a-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihotes cuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett?Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) and response surface methodology (RSM) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 g/l and maltose 3 g/L were most effective for maximum production of enzyme (49 U/ml). The results show a favorable a-amylase production by applying statistical approach in optimization process.

Keywords : a-amylase; response surface methodology; Bitter cassava; Aspergillus niger; Submerge fermentation.