Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

The Comparative Study of Three Chemical Fixatives on Tomato

R. Yang1,2, J.W. Fan2, J.H. Hao1,2 , X.M. Li2, J. Song2, R. Jiang1, K.F. Fang1,2, F.K. Zhao3 and S.H. Wang2*

1Beijing Key Laboratory of New Technology in Agricultural Application, the Beijing University of Agriculture. No. 7 Bei NongRoad, Changping District, Beijing, 102206, P. R. of China. 2College of Plant Science and Technology, the Beijing University of Agriculture. No. 7 Bei NongRoad, Changping District, Beijing, 102206, P. R. of China. 3Biological Science and Technology College, the Beijing University of Agriculture. No. 7 Bei NongRoad, Changping District, Beijing, 102206, P. R. of China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

Cytological study is an important basis to analyze tomato grafting body healing. However, sample fixed is a key step to begin cytology study. Fixative type, concentration, fixed time and its temperature will be affected by the result of tissue fixation, so further influence the subsequent experiments. This paper observes different fixatives on tomato stem cells and cell inclusions of fixed effects using a simple\direct hand sectioning with spontaneous fluorescence. The fixed effect of 2.5% glutaraldehyde fixation fluid of tomato stem is the best method in the three that the?cellular?structure?of?all tissues remained intact under light microscopic and complete chloroplast aglow phenomenon, a spontaneous fluorescence, can be observe in cells. The fixed effect of 50% alcohol fixed fluid to stem is poorer, and there is no cell fluorescence phenomenon through spontaneous fluorescence, and the autofluorescence of cuticular layer and epidermal hair also decreased significantly. The chloroplasts in cells can be fixed by the FAA fixed liquid, and through the cuticular layer and epidermal hair spontaneous fluorescence observation were not weakened, but the fixed effects of cell wall are not better compared with glutaraldehyde fixation fluid. When fixed plant stem, therefore, consider using 2.5% glutaraldehyde fixation fluid is very necessary.

Keywords : Tomato; Fixative; Spontaneous fluorescence.