Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Studies on the Allelopathy of Triterpene Sapogenin from Pistacia chinesis Bunge to Oncomelania hupensis

Shangguang Xia1, Yong Zhang1,2* and Wanxian Wang3

1Anhui Academy of Forestry, Hefei 230022, China. 2School of Environment and Energy Engineering, Anhui University of Architecture, Hefei 230022, China. 3Faculty of Life Science, Hubei University, Wuhan 430062, China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

In this study, we analyzed allelopathic effects of Pistacia chinesis Bunge on Oncomelania hupensis through triterpene sapogenin, a potential molluscicide. The snails were exposed to six different concentrations (0, 2, 5, 10, 20, and 40 mg/L) of triterpene sapogenin and under five periods (24 h, 48 h, 72 h, 96 h, and 120 h). The mortality of snails was positively correlated with the concentration of triterpene sapogenin and exposure time. The liver and intestine observation through transmission electron microscope displayed that 10 mg/L triterpene sapogenin caused apparent damages to the structure of O. hupensis liver. The esterase (EST) isozyme activity of O. hupensis treated by 10 mg/L triterpene sapogenins was higher than that of control between 24-48 h, then decreased after 72 h. It implicates that the extracted triterpene sapogenin from Pistacia chinesis Bunge is promising for controlling the snail. Meanwhile, it provides the foundation for constructing plant community of Pistacia chinesis to control O. hupensis.

Keywords : Pistacia chinesis Bunge, Oncomelania hupensis, Triterpene sapogenin, Molluscicidal activity.