Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

The Research of Selenium Speciation in Se-enriched Kelp by HPLC-ICP-MS

Weisen Yu1, Zhenhua Yu1*, Jie Zhang2** and Qian An1

1Qingdao Center for Disease Control and Prevention, Qingdao 266033, China 2Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

Received on 12 April 2014 and accepted on 09 May 2014



The speciation of selenium in selenium-enriched kelp (Laminaria japonica) was studied by using chromatographic separation coupled with mass spectrometry detection (HPLC-ICP-MS).The kelp was cultivated hydroponically for 60 hours in seawater containing selenite (200 mg/dm3 Na2SeO3) and N and P (150 mg/ dm3 NaNO3 + 25 mg/ dm3 NaH2PO4) nutrient substance. Biotransformation of selenium in the kelp was studied by measuring the concentration of selenium in biological samples. The Selenium-enriched kelp samples were divided into upper, middle and lower parts,and the Selenium values in different parts (dry weight) were 2.26mg/g, 2.29mg/g and 1.89mg/g respectively. The different parts were extracted with water and methanol (90:10), then shook automatically for 24h, selenium speciation was carried out by high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The result was that different organic Se was transformed from inorganic Se in different parts of kelp samples. The species of Se in upper, middle and lower parts in kelp samples were found as three, five and six respectively. Some organic Se was unknown because lack of standards, and further study was needed to identify their structure by high performance liquid chromatography coupled with electro-spray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS).

Keywords : HPLC-ICP-MS; kelp; Selenium, Speciation.