Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Isolation of an Organophosphorus-degrading Strain Pseudomonas sp. Strain YF-5 and Cloning of mpd Gene from this Strain

Zhiyuan Liu1,2,3, Jianfei Xie1,2,?, Yi Shi1,3 and Xin Chen1,3*

1Institute of Applied Ecology, Chinese Academy of Sciences , No.72, Wenhua Road, Shenhe District, Shenyang, Liaoning, China. 2University of Chinese Academy of Sciences, No.19A, Yuquan Road, Beijing, China. 3State?Key?Laboratory?of?Forest?and?Soil?Ecology, China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

Organophosphorus compounds (OP compounds) are toxic, because they can inhibit the acetylcholine esterase in central nervous system of human and animals. OP compounds are extensively used for insecticides control. Water, soil and agricultural products have been contaminated seriously by OP compounds in some regions. It is urgent to find effective methods to remove OP compounds contamination. In this study, an organophosphorus-degrading bacterium (strain YF-5) was isolated from sludge. Based on the results of phenotypic features and phylogenetic similarity of 16S rRNA gene sequences, strain YF-5 was identified as Pseudomonas sp. In liquid culture, 100 mg l-1 methyl parathion and chlorpyrifos were decomposed completely by strain YF-5 (106 cells mL-1) within 8 h and 20 h, respectively. The methyl parathion degrading (mpd) gene was cloned from strain YF-5. Result of sequence BLAST indicated that this gene has 99% similarity to mpd of Plesiomonas sp. M6.

Keywords : Isolation, Pseudomonas sp, Organophosphorus, Degrading, mpd.