Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Influence of Ultrasonic Intensity on Microcystis sp. Viability: A Flow Cytometric Analysis

Gongduan Fan1*, Jing Luo2, Jieyun Chen3, Qian Lin1, Liru Chen1 and Rujing Lin1

1College of Civil Engineering, Fuzhou University, Fuzhou, Fujian Province 350108, China. 2Fujian Jinzhongmin Environment Frotection and Energy-saving Engineering Co., Ltd., Fuzhou, Fujian Province 350000, China. 3Chongqing Jianlong Environmental Protection Engineering Co., Ltd., Chongqing 400000, China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

Cyanobacteria belong to the photosynthetic bacteria by some characteristics of algae. It has long been found that some cyanobacteria may produce toxic hazardous, thus posing a threat to both animals and human health. The green ultrasound technique has been a promising tool for removing cyanobacteria from water while research on the influence of ultrasound on Microcystis sp. viability has attracted wide attention. The effect of ultrasonic intensity on Microcystis sp. viability in this study was investigated while the quantitative analysis of the Microcystis sp. viability during ultrasound was carried out with flow cytometry (FCM). Results indicated that with a lower ultrasonic intensity below the cavitation threshold, 62.23% of the algal cells were damaged but the death rate of algal cells was 0.82%. However, higher ultrasonic intensity above the cavitation threshold led to a death rate up to 41.51% and damage to algal cells of 58.16%.

Keywords : Flow cytometry (FCM), Ultrasound; Cell viability; Microcystis sp., Ultrasonic intensity.