Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Evaluation the Antitumor Effects of Exopolysaccharide Produced by Newly Isolated Bacillus amyloliquefaciens Strain C-1

Wenjuan Luo1, Yin Wan3, Ruijuan Zhang1,2, Haixia Yang1,2 and Bei Han1,2*

1School of Public Health, Xi?an Jiaotong University, Xi?an - 710061, China. 2Nutrition and Food Safety Engineering Research Center of Shaanxi Province, Xi?an - 710061, China. 3State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang - 330047, China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

Exopolysaccharide (EPS) was extracted and purified from a newly isolated Bacillus amyloliquefaciens strain, C-1. The anti-tumor activities of this EPS were tested in vitro. C-1 EPS exhibited good and direct, dose-dependent in vitro anti-proliferative effects on 6 human cancer cell lines. The inhibition rates of 7 mg/ml EPS on tumor cells SGC-7901, HeLa, A549, 7721, 7402, and MCF-7 were 73.2%, 78.6%, 65.0%, 64.0%, 41.1%, and 57.7%, respectively. The anti-proliferative effect of EPS was associated with the direct induction of apoptotic cell death, rather than the cell cycle. It was also verified that the expression of anti-apoptotic Bcl-2 family proteins was decreased. These results collectively show that this EPS has considerable potential for use in medical compounds.

Keywords : Exopolysaccharides; Anti-tumor activity; Cell apoptosis; Bacillus amyloliquefaciens C-1.