Journal of Pure and Applied MicrobiologyVol. 8 No. May 2014 Special Edition

Effects of Long-term Application of Fertilizers on Microbial Community Structure and Diversity of Tomato Rhizosphere Soil

Jinghong Hao1,a, Ningning Ma2,a, Shuangxi Fan1, Rui Yang1, Zhonghua Ren1, Jiarui Zhao1, Lu Wang1, Jiaxing Wang1, Weigen Li1, Gaoyang Pan1, Zhuhui Liu1, Pengfei Sheng1 and Tianlai Li3,1*

1Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, the Beijing University of Agriculture. No. 7 Bei Nong Road, Changping District, Beijing, P. R. of China. 2Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences. No. 72 Wen Hua Road, Shenhe District, Shenyang, P. R. of China. 3Key Laboratory of Protected Horticulture of Education Ministry in Liaoning Province, College of Horticulture, Shenyang Agricultural University, No. 120 Dong Lin Road, Shenhe District, Shenyang, P.R. China.

Received on 12 April 2014 and accepted on 09 May 2014

 

ABSTRACT

Excessive use of chemical fertilizer, unbalanced application of nitrogen, phosphorus and potassium, usually lead to lower utilization of soil fertilizer, decline in the quality of the ecological environment, serious pests and diseases, decrease yield and quality of vegetables. Soil microbiological characteristic is an important characterization of soil quality and can reflect the soil fertility condition. To investigate the effect of fertilizer on soil microorganism, tomato (Lycopersicon esculentum L. Liaoyuanduoli) was used as material, an experimental vegetable field which had 20 years of cultivation history and had been under long-term localized fertilization was used as a platform to analyse the soil microbiological characteristics after different fertilization treatments using PCR-DGGE technology. The results showed that the organic fertilizer application could improve the soil microbial quantity, increase the number of dominant species, increase the dominant population number, stable community structure. Excessive use of nitrogen fertilizer could change the species of soil bacteria dominant populations. Different kinds of chemical fertilizer or fertilizer combinations had no effects on soil microorganism community structure.

Keywords : Tomato; Fertilizer; Microbial community; Structure and diversity; Rhizosphere soil.