Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Production Improvement of Epothilone B by Sorangium cellulosum by Mutation of Strain and Optimization of the Fermentation Process

Guoli Gong*, Na Wang and Lili Liu

College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi?an - 710 021, China.

Received on 26 November 2013 and accepted on 09 January 2014

 

ABSTRACT

With the aim of obtaining the high producing strain of epothilones B, we mutated Sorangium cellulosum SoF5-76 with the combination treatment of UV rays and N-Methyl-N?-Nitro-N-Nitrosoguanidine (NTG) to improve the yields. Epothilone B yield was improved from 35.24mg/L to 79.83 mg/L of the broth resulting in a high yielding mutant. Then, Plackett-Burman design and the response surface methodology (RSM) were applied to optimize fermentation process of the high producing mutant. The obtained optimal fermentation conditions were potato starch 4.8 g / L, skim milk powder 2.3g / L, glucose 0.5g / L, soybean powder 2g / L, magnesium sulfate 2g / L, chlorine calcium 2g / L, EDTA-Fe3 + 2mL / L, trace elements 0.5mL / L, absorbent resin 2%, pH7.4, liquid volume 50mL/250mL, inoculation amount 8%, temperature 30?C. Under this optimal condition, epothilone B production reached up to 108.67mg / L that was 36.13% higher than preliminary conditions. It is the higher yield of epothilone B in the epothilone producing strains reported in literatures.

Keywords : Sorangium cellulosum, Epothilone B, Mutation, Optimization, Response surface methodology.