Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Isolation and Rapid Identification of a Kerala Isolate of Duck Enteritis Virus using Polymerase Chain Reaction

S. Aravind, N.M. Kamble, S.S. Gaikad, S.K. Shukla, S.A. Khulpae, Sohini Dey and C. Madhan Mohan*

Recombinant DNA Laboratory, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh - 243122, India.

Received on 08 July 2013 and accepted on 19 October 2013

 

ABSTRACT

Duck enteritis virus (DEV) is a herpes virus that cause an acute, contagious, and fatal disease. In this present article, we introduce a polymerase chain reaction (PCR) assay for DEV DNA. The method employed primer sets targeting the viral DNA polymerase gene (UL30), type I membrane protein that binds to heparin sulphate (glycoprotein C) and glycoprotein E of DEV and was able to amplify DNA fragments of the expected size from infected samples. The method will provide a valuable tool for a rapid laboratory diagnosis of DEV infection. By virtue of its high throughput format, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency of the virus.

Keywords : Duckenteritis virus, DNA polymerase, Glycoprotein C, Glycoprotein E Polymerase Chain Reaction.