Journal of Pure and Applied MicrobiologyVol. 8 No. 2

In vitro and In vivo Biocontrol Performance of Trichoderma harzianum Rifai on Ganoderma boninense Pat. Related to Pathogenicity on Oil Palm (Elaeis guineensis Jacq.)

Laila Naher1,2, Umi Kalsom Yusuf2, Soon Guan Tan3, Shafiquzzaman Siddiquee4 and M. Robiul Islam5

1Faculty of Agro Based Industry, Universiti Kelantan Malaysia,17600 Jeli, Kelantan. 2Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. 3Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. 4Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia. 5Department of Agronomy and Agricultural Extension, University of Rajshahi, Rajshahi 6205, Bangladesh.

Received on 18 November 2013 and accepted on 10 January 2014



Ganoderma boninense, causes basal stem rot disease in oil palm, which leads to severe losses to the palm oil industry. The antagonistic effect of Trichoderma harzianum on G. boninense (a causal pathogen for oil palm) was tested by in vitro and in vivo studies. T. harzianum inhibited the mycelia growth of G. boninense on in vitro plate assay experiment and in vivo on the glass house grown oil palm plants. During the in vivo experiment, at first time point after one month, G. boninense development was found in oil palm root tissues in control plants whereas in T. harzianum treated plants no disease symptoms were observed until at the end of the experiment. It is a broad spectrum approach to use T. harzianum to control G. boninense infection of oil palm.

Keywords : In vitro, In vivo, Biocontrol, Trichoderma, Ganoderma, Oil palm.