Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Evaluation of Antioxidant, Antiradicalic and Antimicrobial Activities of Kernel Date (Fructus dactylus)

Hulya Celik1, Kaan Kucukoglu2, Hayrunnisa Nadaroglu3* and Merve Senol4

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Agr? Ibrahim Cecen University, 04000Agri, Turkey. 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ataturk University, 25240 Erzurum, Turkey. 3Department of Food Technology, ErzurumVocational Training School, A Taturk University, 25240 Erzurum, Turkey. 4Department of Biotechnology, Agriculture Faculty, Ataturk University, 25240 Erzurum, Turkey.

Received on 10 June 2013 and accepted on 12 August 2013

 

ABSTRACT

In order to determine the antioxidant and radical scavenging properties of kernel date (Fructus dactylus) the following analysis were conducted: The total antioxidant activity via the ferric thiocyanate method; 2,2'-azinobis-(3-ethylbenzothiazole-6-sulphonate) (ABTS) radical scavenging activity; superoxide anion radical (O2?-) scavenging activity; the total reduction power through potassium ferricyanide reduction method; Cupric ions (Cu2+) reduction capacity through Cuprac method; hydrogen peroxide scavenging activity and chelating activity of ferrous ions (Fe2+). Additionally, total phenolic and flavonoid contents of the kernel of date (KD) (Fructus dactylus) were determined. Furthermore, a-tocopherol, butylated hydroxyanisole (BHA) and quercetin were used as the reference antioxidant compounds. Both KD-Aquous extract and KD-ethanol extract exhibited the highest phenol (KD-AE:107; KD-EE237 ?g GAE mg-1 extract) and flavonoid (KD-AE:210; KD-EE:236 ?g QE mg-1 extract) contents and displayed the highest antioxidant activity. KD-AE and KD-EE exhibited high antibacterial activitiy against eleven bacteria with Minimum Inhibitory Concentrations (MIC) values ranging from 12.50 to 250 mu l/ml.

Keywords : Antioxidant activity, Radical scavenging, Phenolic derivatives, Antibacterial activity.