Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Assessment of Some Physiological Parameters and HAK1-type Transporter Transcriptional Changes in Aeluropus littoralis under NaCl Stress

Masoumeh Rezaei Moshaei1*, Ghorban Ali Nematzadeh1, Hossein Askari2, Leila Haghighi3 , Amir Sasan Mozaffari Nejad4 and Ali Asghar Ahmadi5

1Department of Agronomy and Plant Breeding, Agricultural sciences & Natural Resources University, Sari, Iran. 2Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran. 3Department of Biology, Kharazmi University, Tehran, Iran. 4Young Researchers and Elite Club, Sari Branch, Islamic Azad University, Sari, Iran. 5North Research Center-Pasteur Institute of Iran, Amol, Iran.

Received on 02 August 2013 and accepted on 13 October 2013

 

ABSTRACT

Maintenance of internal status of K+ ion is one of the important mechanisms for regulation of potassium balance under salt stress. In the present study, we use Aeluropus littoralis as a halophytic monocot model to investigate transcriptional changes in HAK1 gene that code a H+-K+transporter for acquire potassium under condition of limited availability of K+ and also measurement of some physiological parameters such as Dry weight, Ash and K+ content, Photosynthetic pigments and Carbohydrate content under salt stress. The Comparison of HAK1 gene expression in pattern in shoot and root revealed that transcript levels of HAK1 in shoot induced by salt androot contained less levels of HAK1 mRNA than shoot at high concentrations of NaCl. Our results suggest that HAK1-type transporters can play an important role in potassium uptake and cell internal diffusion of K+ ion to tissue that can be effective in salt tolerance in this grass.

Keywords : Photosynthetic pigments, Soluble sugar and starch, Semi?quantative RT-PCR, K+transporter, Salinity.