Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Microbiological Pattern and Antibiotic Susceptibility of Agents Isolated from Nosocomial Infections, Staff and Equipment of Surgery Section and ICU of Imam Khomaini Hospital, Ilam, Iran

Mirta Azizi1, Iraj Pakzad1,4*, Zahra Karam Nejad2,3, Fariba Khodayari2,3, Reza Azizian1,4, Farid Azizi Jalilian4, Morevat Taherikalani4, Masoud Hamidi6 and Reza Pakzad6

1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Iran. 2 Student Research Committee, Ilam University of Medical Sciences, Iran. 3Department Nursing, Faculty of Nursing and Midwifery, Ilam University of Medical Sciences, Iran. 4Department Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran. 5Department of Pharmaceutical Biotechnology, Tabriz University of Medical Sciences, Iran. 6Department of Epidemiology, Medical School, Tehran University of Medical Sciences, Tehran, Iran.

Received on 11 June 2013 and accepted on 20 August 2013



Resistant bacteria in various sections of hospital as a main issue of infection transmission is undeniable. Thereby, to achieve infection disease control on hospital, bacteria recognizing and antibiotic resistance determination is necessary. This study is aimed to determine the pattern of microbial resistant of isolates that isolated from surgery ward and ICU and compare it with osocomial infection isolates. In a descriptive and cross-sectional study, during 6 months sampling was carried out from devices and ersonnel of surgery and ICU ward. In same period Nosocomial infections from surgery and ICU wards were cultured. Bacterial on Standard criteria according to CLSI were diagnosed. Antibiotic susceptibility were done according to Kirby-Bauer Disk Diffusion .After culturing 130 samples were positive, the most frequency of isolated bacteria in both wards were Staphylococcus saprophyticus. Most frequent bacteria in ICU and Surgery ward involved; Entrobacter (35%) and E.coli (25%), in a row. In nosocomial infections, frequency of samples that cultured were positive included in; Lesion (45%), Sputum(42.5%) and Urine(12.5%). E.coli, Klebsiella and Staphylococcus aureus isolated from ICU shown high resistance to Amplicillin, Ceftazidime/Cefotaxime , Ceftriaxone, respectively. Whereas bacteria that isolated from Surgery ward shown high resistance to Tetracycline, Amoxicillin- Tetracycline, Ceftriaxone. Regarding to resistance pattern , there is a correlation between resistance of species that isolated from personnel and devices with nasocomial isolates. Therefore, it could be concluded that devices and personnel have main role to disseminate infection. Thereby, the proper disease control policy could be so useful to combat with issue.

Keywords : Antibiotic susceptibility, Resistant Bacteria, ICU, Surgery, Hospital acquired infection.