Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Effect of Cell Suspension-Alginate Ratio, Tween 80 and Oil-Water Ratio on Microcapsulation of B. bifidum BB01 and BB28

He Chen, Yajuan Song, Ye Wang and Guowei Shu*

College of Life Science & Engineering, Shaanxi University of Science & Technology, Xi?an, China.

Received on 11 October 2013 and accepted on 07 January 2014

 

ABSTRACT

There are many reports on the study of microencapsulated bifidobacteria, the effect of concentration of sodium alginate, emulsifying time and immobilized time on microcapsulation of B. bifidum BB28 and BB01 have been invesgated. This study reported the viable counts and encapsulation yield (EY) of B. bifidum BB01 and BB28 encapsulated in different cell suspension-alginate ratios (1:5, 1:10, 1:15 and 1:20), in different Tween 80 content(0.2%, 0.4%, 0.6% and 0.8%) and in different oil-water ratios (1:4, 1:5, 1:6 and 1:7). It was studied by single factor experiment method, the results showed that this several factors impacted the viable counts and encapsulation yield (EY) of B. bifidum BB01and BB28 significantly, and the optimum cell suspension-alginate ratios for B. bifidum BB01 and BB28 were 1:5 and 1:10 respectively; the optimum Tween 80 concentration for B. bifidum BB01 and BB28 were 0.8% and 0.6% respectively; the optimum oil-water ratios were 1:6 and 1:4 respectively.

Keywords : Alginate, Bifidobacterium bifidum, Microencapsulation, Emulsion.