Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Investigations on the Inhibition Effects of 1,8-cineole against Microbial Biofilms

Lin Wang1, Jianhua Zhang2, Fengxiang Wei3, Xiaoguang Liu2, Meili Yang2, Xue Dong2, Hui Wang2, Yan Liu2, Zhiwei Yang2* and Junxing Liu2*

1Department of Laboratory Medicine, The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, China. 2Department of Pathophysiology, School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China. 3The Genetics Laboratory, Maternity and Child Healthcare Hospital, Longgang District, Shenzhen 518172, China.

Received on 25 September 2013 and accepted on 06 December 2013



Bacteria living as biofilm are frequently reported to exhibit the resistances to the common antimicrobial agents. Hence, the in vitro antimicrobial activities of the compound 1,8-cineole were evaluated to explore the novel agent. The bioactivities were estimated by the MIC and MBC determinations on 8 species of bacteria and fungi, as well as the confocal laser scanning on 3 types of bacterial biofilms. It was found that the essential oil has good inhibiting activities against fungi rather than bacteria, especially Candida albicans, with the lowest MIC value (0.156 %). Both of gram-positive and gram-negative bacterial biofilms are sensitive to 1,8-cineole. In addition, there is linear relationship between inhibition activities and concentration of 1,8-cineole. Further analysis revealed that 1,8-cineole can prompt the biofilm-surface bacteria to die off or live as planktonic, in order to help the immune system to remove the bacteria. Thus, 1,8-cineole could be judged as a kind of potential drug with rather antimicrobial activities.

Keywords : 1,8-cineole; Antimicrobial activities; Bacterial biofilms; Confocal laser scanning.