Journal of Pure and Applied MicrobiologyVol. 8 No. 2

Study of Fusarium equiseti, As Bicontrol Agent of Echinochloa oryzicola in Paddy Fields

Mohammad Reza Safari Motlagh*

1Department of Plant Pathology, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran.

Received on 18 February 2014 and accepted on 21 April 2014



Echinochloa oryzicola is an obligate weed with an elaborated survival strategy in the flooded rice. Fungal pathogens can be exploited as biological agents for the management of agricultural weeds. In this research, Fusarium equiseti (Corda) Saccardo was isolated from Echinochloa oryzicola and studied as a biocontrol agent against this weed in paddy fields. Pathogenicity test of isolates revealed the pathogenicity of this fungus and its ability to cause leaf spot and leaf blight on Echinochloa oryzicola. Inoculation was done using a spore suspension consisting of 105 spore/ml distilled water at the 2-3 leaf stage. Also, inhibition of seeds germination test was done. The results showed that Fusarium equiseti able to infect Echinochloa oryzicola in a higher rate comparing with rice cultivar. Moreover, the results indicated that seeds germination of Echinochloa oryzicola was significantly affected by Fusarium equiseti in comparison with controls. Hence Fusarium equiseti can be considered as a probable bioherbicide for controlling of Echinochloa oryzicola at the 2-3 leaf stage of growth of weed.

Keywords : Echinochloa oryzicola, Fusarium equiseti, biological control.